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fgf4  (R&D Systems)


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    Structured Review

    R&D Systems fgf4
    Fgf4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+fgf4/Recombinant+Mouse+FGF-4+(aa+67-202)+Protein/pm41339717-430-26-27
    Average 93 stars, based on 7 article reviews
    fgf4 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: NODAL Secures Pluripotency upon Embryonic Stem Cell Progression from the Ground State
    Article Snippet: .. DE1 comprises batch-tested N2B27, supplemented with 3 mM Chir99021, 20 ng/mL activin A, 10 ng/mL recombinant mouse FGF4 (R&D Systems, 235-F4-025), 1 mg/mL heparin (SigmaAldrich, H3393), and 100 nM PI103 (Cayman Chemical, 10009209). .. DE2 medium is composed of SF5 base (DMEM/F12 [Life Technologies, 21331-020], 0.25% N2, 1% B27 without RA [Life Technologies, 12587-010], 0.05% BSA [Life Technologies, 15260-037], 0.1 mM b-mercaptoethanol [Life Technologies, 31350-010], 2 mM L-glutamine [Life Technologies, 25030-081]), supplemented with 3 mM Chir99021, 20 ng/mL activin A, 10 ng/mL FGF4, 1 mg/mL heparin, 100 nM PI103, and 20 ng/mL epidermal growth factor (EGF; Preprotech, AF-100-15).

    Article Title: Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro
    Article Snippet: Heparin (Sigma-Aldrich, cat. no. H3149–25KU). .. Recombinant mouse Fgf4 (aa 67–202) protein (R&D Systems, cat. no. 7486-F4-025). .. RPMI-1640 (Thermo Fisher Scientific, cat no. 21870076).

    Article Title: Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro.
    Article Snippet: The interaction between embryonic and extraembryonic tissues is critical in natural mouse embryogenesis.. Here, to enable such interaction in vitro, we describe a protocol to assemble a complete mouse embryo model using mouse embryonic stem cells and induced embryonic stem cells to express Cdx2 (or trophoblast stem cells) and Gata4 to reconstitute the epiblast, extraembryonic ectoderm and visceral endoderm lineages, respectively.. The resulting complete embryo models recapitulate development from embryonic day 5.0 to 8.5, generating advanced embryonic and extraembryonic tissues that develop through gastrulation to initiate organogenesis to form a head and a beating heart structure as well as a yolk sac and chorion.

    Article Title: Centimeter-scale quorum sensing dictates collective survival of differentiating embryonic stem cells
    Article Snippet: .. After 2 days of culturing in N2B27, we added 500 nM of Retinoic Acid, and one or combinations of the following recombinant proteins to the medium: 200 ng/mL of recombinant mouse FGF4 (R&D Systems, #7486-F4), 200 ng/mL of recombinant human FGF5 (R&D Systems, #237-F5), 100 ng/mL of recombinant mouse PDGFA (Novus, NBP1-43148), 100 ng/mL of recombinant mouse VEGFB 186 (Novus, #767-VE), 100 ng/mL of recombinant mouse VEGFA (Novus, #493-MV), 500 ng/mL of recombinant human CYR61/CCN1 (Novus, #4055-CR), 500 ng/mL of recombinant human CTGF/CCN2 (Novus, #9190-CC), 200 ng/mL of recombinant mouse CLU (Novus, #2747-HS), 500 ng/mL of recombinant human HSPA8/HSC70 (Novus, #NBP1-30278), 1000 ng/mL of recombinant human Cyclophilin A (PPIA) (Novus, #NBC1-18425), or 2000 ng/mL of mouse recombinant SCF (STEMCELL, #78064). ..

    Article Title: Macroscopic quorum sensing sustains differentiating embryonic stem cells
    Article Snippet: .. After 2 days of culturing in N2B27, we added 500 nM of RA and one or combinations of the following recombinant proteins to this medium: 200 ng ml −1 of recombinant mouse FGF4 (R&D Systems, 7486-F4), 200 ng ml −1 of recombinant human FGF5 (R&D Systems, 237-F5), 100 ng ml −1 of recombinant mouse PDGFA (Novus, NBP1-43148), 100 ng ml −1 of recombinant mouse VEGFB 186 (Novus, 767-VE), 100 ng ml −1 of recombinant mouse VEGFA (Novus, 493-MV), 500 ng ml −1 of recombinant human CYR61/CCN1 (Novus, 4055-CR), 500 ng ml −1 of recombinant human CTGF/CCN2 (Novus, 9190-CC), 200 ng ml −1 of recombinant mouse CLU (Novus, 2747-HS), 500 ng ml −1 of recombinant human HSPA8/HSC70 (Novus, NBP1-30278), 1,000 ng ml −1 of recombinant human cyclophilin A (PPIA) (Novus, NBC1-18425) or 2,000 ng ml −1 of mouse recombinant SCF (STEMCELL Technologies, 78064). ..

    Article Title: Macroscopic quorum sensing sustains differentiating embryonic stem cells.
    Article Snippet: .. After 2 days of culturing in N2B27, we added 500 nM of RA and one or combinations of the following recombinant proteins to this medium: 200 ng ml−1 of recombinant mouse FGF4 (R&D Systems, 7486-F4), 200 ng ml−1 of recombinant human FGF5 (R&D Systems, 237-F5), 100 ng ml−1 of recombinant mouse PDGFA (Novus, NBP1-43148), 100 ng ml−1 of recombinant mouse VEGFB 186 (Novus, 767-VE), 100 ng ml−1 of recombinant mouse VEGFA (Novus, 493-MV), 500 ng ml−1 of recombinant human CYR61/CCN1 (Novus, 4055-CR), 500 ng ml−1 of recombinant human CTGF/CCN2 (Novus, 9190-CC), 200 ng ml−1 of recombinant mouse CLU (Novus, 2747-HS), 500 ng ml−1 of recombinant human HSPA8/HSC70 (Novus, NBP1-30278), 1,000 ng ml−1 of recombinant human cyclophilin A (PPIA) (Novus, NBC1-18425) or 2,000 ng ml−1 of mouse recombinant SCF (STEMCELL Technologies, 78064). ..

    Article Title: NODAL Secures Pluripotency upon Embryonic Stem Cell Progression from the Ground State
    Article Snippet: .. DE1 comprises batch-tested N2B27, supplemented with 3 μM Chir99021, 20 ng/mL activin A, 10 ng/mL recombinant mouse FGF4 (R&D Systems, 235-F4-025), 1 μg/mL heparin (Sigma-Aldrich, H3393), and 100 nM PI103 (Cayman Chemical, 10009209). .. DE2 medium is composed of SF5 base (DMEM/F12 [Life Technologies, 21331-020], 0.25% N2, 1% B27 without RA [Life Technologies, 12587-010], 0.05% BSA [Life Technologies, 15260-037], 0.1 mM β-mercaptoethanol [Life Technologies, 31350-010], 2 mM L-glutamine [Life Technologies, 25030-081]), supplemented with 3 μM Chir99021, 20 ng/mL activin A, 10 ng/mL FGF4, 1 μg/mL heparin, 100 nM PI103, and 20 ng/mL epidermal growth factor (EGF; Preprotech, AF-100-15).

    Cell Culture:

    Article Title: Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro.
    Article Snippet: The interaction between embryonic and extraembryonic tissues is critical in natural mouse embryogenesis.. Here, to enable such interaction in vitro, we describe a protocol to assemble a complete mouse embryo model using mouse embryonic stem cells and induced embryonic stem cells to express Cdx2 (or trophoblast stem cells) and Gata4 to reconstitute the epiblast, extraembryonic ectoderm and visceral endoderm lineages, respectively.. The resulting complete embryo models recapitulate development from embryonic day 5.0 to 8.5, generating advanced embryonic and extraembryonic tissues that develop through gastrulation to initiate organogenesis to form a head and a beating heart structure as well as a yolk sac and chorion.

    Modification:

    Article Title: Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro.
    Article Snippet: The interaction between embryonic and extraembryonic tissues is critical in natural mouse embryogenesis.. Here, to enable such interaction in vitro, we describe a protocol to assemble a complete mouse embryo model using mouse embryonic stem cells and induced embryonic stem cells to express Cdx2 (or trophoblast stem cells) and Gata4 to reconstitute the epiblast, extraembryonic ectoderm and visceral endoderm lineages, respectively.. The resulting complete embryo models recapitulate development from embryonic day 5.0 to 8.5, generating advanced embryonic and extraembryonic tissues that develop through gastrulation to initiate organogenesis to form a head and a beating heart structure as well as a yolk sac and chorion.



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    Image Search Results


    Journal: Cell reports

    Article Title: Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis

    doi: 10.1016/j.celrep.2024.114789

    Figure Lengend Snippet:

    Article Snippet: Recombinant Mouse FGF4 Protein , R&D Systems , 5846-F4.

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Catecholamine ELISA, Clinical Proteomics, Sandwich ELISA, Dehydrogenase Assay, Activity Assay, Software